Journal: Cell Reports Medicine

Article Title: L858R emerges as a potential biomarker predicting response of lung cancer models to anti-EGFR antibodies: Comparison of osimertinib vs. cetuximab

doi: 10.1016/j.xcrm.2023.101142

Figure Lengend Snippet: Cetuximab inhibits relapses of animal models carrying either L858R-EGFR (11-18) or an overexpressed Del19-EGFR (HCC827), but a model expressing normal levels of Del19-EGFR (PC9) does not respond to cetuximab 11-18 (A, 1 × 10 7 cells/mouse), PC9 (B, 3 × 10 6 /mouse), or HCC827 cells (C, 4 × 10 6 /mouse) were subcutaneously implanted in the flanks of CD1-nu/nu mice. When tumors became palpable, mice were randomized in groups of 5–7 animals (each) that were treated (gray areas) for 58 days (A) or 21 days (B and C) with cetuximab (0.2 mg/mouse/injection) once every 3 days, or daily with either osimertinib (10 mg/kg/day in A or 5 mg/kg in C) or erlotinib (50 mg/kg/day in B). Shown are tumor volumes of individual mice from each group. Animal numbers per group are indicated (N). See also Figures S5 and .

Article Snippet: CD1 nude mice (HsdHli:CD1-Foxn1nu) , Envigo Israel , N/A.

Techniques: Expressing, Injection

Journal: Cell Reports Medicine

Article Title: L858R emerges as a potential biomarker predicting response of lung cancer models to anti-EGFR antibodies: Comparison of osimertinib vs. cetuximab

doi: 10.1016/j.xcrm.2023.101142

Figure Lengend Snippet:

Article Snippet: CD1 nude mice (HsdHli:CD1-Foxn1nu) , Envigo Israel , N/A.

Techniques: Derivative Assay, Recombinant, Generated, Transfection, Plasmid Preparation, Software

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Mmp-9 is expressed in mice injured skin tissues ( a–d ) and in keratinocytes ( e–j ). Mmp-9 mRNA expression in the skin of nude (Hsd:Athymic Nude-Foxn1 nu ) and BALB/c mice at post-wounding days 1, 3, and 5 ( a ). Immunofluorescent detection of Foxn1-eGFP ( b ) and Mmp-9 ( c ) and co-localization of Foxn1-eGFP and Mmp-9 signals ( d ) in the skin of Foxn1::Egfp mice at day 2 after injury. Mmp-9 mRNA ( e , g ) and corresponding Mmp-9 protein ( f , h ) expression in keratinocytes isolated from B6 (E-H, J), BALB/c ( i ) and nude ( i ) mice and co-cultured with dermal fibroblasts (DFs). Keratinocytes were transfected with Foxn1-containing plasmid ( e , f ) or adenovirus (Ad-Foxn1; g–j ) and cultured for 48 h. Control cultures were transfected with a vector expressing GFP alone. Representative Western blot analysis of phospho-PKC (pan) protein expression in Ad-Foxn1- or Ad-GFP-transfected keratinocytes co-cultured with DFs ( j ). Full-length blots and densitometric analysis are presented in Supplementary Fig. . ( b–d ) Scale bar 50 µm. Values are the mean ± SD, ****p < 0.0001. ( a ) Modified from: Scarless skin wound healing in FOXN1-deficient (nude) mice is associated with distinctive matrix metalloproteinase expression. Gawronska-Kozak B., Matrix Biol 2011;30(4):290–300 , with permission of Elsevier.

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Expressing, Isolation, Cell Culture, Transfection, Plasmid Preparation, Western Blot, Modification

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Foxn1 regulates keratinocyte differentiation and migration. Flow cytometric analysis of keratinocytes: non-transfected and transfected with Ad-Foxn1 or Ad-GFP, showing the percentage expressing keratin 14 (K14) only, keratin 10/keratin 14 (K10/K14) and K10 only ( a ). Representative Western blots of keratin 16 and involucrin protein expression in keratinocytes transfected with Ad-Foxn1 or Ad-GFP (control) ( b ). Full-length blots are presented in Supplementary Figure . Migratory abilities after pre-treatment with mitomycin C and wounding in monolayer cultures were analysed in non-transfected B6 keratinocytes and in keratinocytes transfected with Ad-Foxn1 or Ad-GFP ( c , d ). Representative images were taken at 0, 20, 32 and 68 h ( d ). Dotted lines indicate the distance between migrating keratinocytes ( d ). Migration is expressed as the percentage of the distance between the unclosed edges ( c ). Each well was prepared in duplicate, and the experiment was repeated two times (n = 11 animals). Values are the mean ± SD. Asterisks indicate significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Migration, Transfection, Expressing, Western Blot

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Simplified list of proteins with differential abundance in keratinocytes transfected with Ad-Foxn1 and control keratinocytes (transfected with Ad-GFP).

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Transfection

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Proteins identified both in proteomic analysis as differentially abundant in keratinocytes transfected with Ad-Foxn1 and keratinocytes transfected with Ad-GFP and among genes selected from sequencing data (skin samples of Foxn1-deficient nude mice vs Foxn1-active control mice).

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Transfection, Sequencing

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Foxn1 overexpression in keratinocytes affects proteins that regulate the hypoxia/normoxia response. Schematic representation of molecular cross-talk between proteins exhibiting hypoxic or normoxic characteristics, according to proteome profiling results, and induced in keratinocytes transfected with Ad-Foxn1 (black colour) or Ad-GFP (control; white colour) ( a ). Grey colour indicates proteins that were not detected in the analysis but link identified proteins ( a ). Foxn1 mRNA expression in primary cultures of B6 keratinocytes ( b ) and Ad-Foxn1 or Ad-GFP (control)-transfected nude keratinocytes ( c ) cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions. Corresponding Mmp-9 mRNA ( d ) and Mmp-9 protein ( f ) expression in keratinocytes isolated from B6 mice and Mmp-9 mRNA ( e ) and Mmp-9 protein ( g ) expression in nude keratinocytes transfected with Ad-Foxn1 or Ad-GFP (control) cultured under normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions. Values are the mean ± SD; **p < 0.01, ***p < 0.001, ****p < 0.0001; m – molecular size marker.

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Over Expression, Transfection, Expressing, Cell Culture, Isolation, Marker

Journal: Scientific Reports

Article Title: Foxn1 expression in keratinocytes is stimulated by hypoxia: further evidence of its role in skin wound healing

doi: 10.1038/s41598-018-23794-5

Figure Lengend Snippet: Foxn1 overexpression in keratinocytes affects pro-survival and pro-apoptotic protein profiles. Schematic representation of molecular cross-talk between proteins exhibiting pro- or anti-apoptotic characteristics, according to proteome profiling results, and induced in keratinocytes transfected with Ad-Foxn1 (black colour) or Ad-GFP controls (white colour) ( a ). Grey colour indicates proteins that were not detected in the analysis but link identified proteins ( a ). Representative Western blot ( b ) and densitometric analysis ( c ) (n = 4) of Vdac1 protein expression in keratinocytes transfected with Ad-GFP (control) or Ad-Foxn1. Full-length blots are presented in Supplementary Fig. . Flow cytometric analysis of keratinocytes transfected with Ad-Foxn1 or Ad-GFP (control) showing the percentages of necrotic, viable and apoptotic cells and cells in G0/G1, S or G2/M phase ( d ). The values are the mean ± SD; *p < 0.05, ***p < 0.001; ****p < 0.0001.

Article Snippet: Foxn1-GFP (MG226744) and Foxn1-Ddk (MR226744) plasmid vectors were purchased from OriGene.

Techniques: Over Expression, Transfection, Western Blot, Expressing